Peptide Calculator

Peptide dosing is a three-step calculation. First find the concentration: divide the peptide amount in the vial by the bacteriostatic water you added — for example 10 mg ÷ 2 ml = 5 mg/ml (5,000 mcg/ml). Second, find the volume to draw: divide your target dose by the concentration — a 250 mcg dose ÷ 5,000 mcg/ml = 0.05 ml. Third, convert to syringe units: on a U-100 insulin syringe 1 ml = 100 units, so 0.05 ml = 5 units. The calculator at the top of this page runs all three steps for any peptide, in mcg, mg or IU, and draws the result on a syringe for you.

Concentration (mcg/ml) = peptide amount in mcg ÷ millilitres of bacteriostatic water. Volume to inject (ml) = dose in mcg ÷ concentration in mcg/ml. Syringe units (U-100) = volume in ml × 100. Doses per vial = total peptide in mcg ÷ dose in mcg. Those four lines are the entire maths behind every peptide calculator — this one just does the arithmetic instantly and error-free.

There is no single “correct” amount — the water only sets how concentrated the solution is and how many units you draw. A common choice is to add enough water so your dose lands on a round syringe number. For a 5 mg BPC-157 vial, 2 ml of BAC water gives 2.5 mg/ml, so a 250 mcg dose is a clean 10 units; for a 10 mg vial, 2 ml gives 5 mg/ml so 250 mcg is 5 units. Use the Bacteriostatic-Water tab on this page to reverse-solve the exact ml for any target. As a rule most 5–10 mg research vials are reconstituted with 1–3 ml.

On a standard U-100 insulin syringe the whole 1 ml barrel is 100 units, so each unit is 0.01 ml. To turn a dose in mcg into units: units = (dose in mcg ÷ concentration in mcg/ml) × 100. Example — at 5,000 mcg/ml (10 mg in 2 ml), a 500 mcg dose is (500 ÷ 5,000) × 100 = 10 units. The mcg-to-units converter in the Converters tab does this both ways.

A blend vial holds two peptides at once — most often BPC-157 and TB-500 (the “Wolverine” blend), commonly sold as 5 mg/5 mg or 10 mg/10 mg. Because both peptides share the same liquid, every draw delivers a fixed ratio of each. The Blend tab on this page lets you set one target dose (say 250 mcg of BPC-157) and instantly shows the matching TB-500 dose, the volume, the syringe units and how many doses the blend vial contains. It works for any two-peptide blend, including CJC-1295 + ipamorelin.

A very common reference protocol for a 5 mg/5 mg blend is to reconstitute with 2–3 ml of bacteriostatic water and take a draw that delivers roughly 250 mcg of each peptide once daily (some run BPC-157 twice daily and TB-500 as a larger 2×-weekly dose instead). With 5 mg + 5 mg in 2 ml, 10 units (0.1 ml) delivers 250 mcg BPC-157 + 250 mcg TB-500. These are commonly-cited research ranges only — not a prescription — so confirm any protocol with a licensed healthcare provider.

The “Wolverine stack” is the popular nickname for combining BPC-157 and TB-500 — two peptides studied for tissue repair — named after the comic-book character’s rapid healing. It is sold either as two separate vials or as a single pre-mixed BPC-157/TB-500 blend. Use the Blend tab to dose a pre-mixed vial, or the main tab twice (once per peptide) if you run them separately.

Commonly-cited reference doses for BPC-157 are 250–500 mcg once or twice daily, run in cycles of 4–8 weeks. At 10 mg reconstituted in 2 ml (5 mg/ml) that is 5–10 units per dose; at 5 mg in 2 ml (2.5 mg/ml) it is 10–20 units. Enter your own vial size and target in the calculator for the exact units. This is general reference information, not medical advice.

A frequently referenced TB-500 protocol is a loading phase of about 2–2.5 mg twice per week for 4–6 weeks, then a smaller maintenance dose every 1–2 weeks. At 10 mg in 2 ml (5 mg/ml), 2.5 mg is 0.5 ml = 50 units. Because TB-500 doses are much larger than BPC-157 doses, blends are usually dosed by the BPC-157 target and the TB-500 amount simply follows the ratio.

Let the vial and the bacteriostatic water reach room temperature. Wipe both rubber stoppers with an alcohol swab. Draw your chosen volume of BAC water into a syringe, then inject it slowly down the inside wall of the vial — aim the stream at the glass, not directly onto the powder. Do not shake; gently swirl or let it sit until the powder fully dissolves (a few minutes). Store the reconstituted vial in the refrigerator. This calculator tells you the resulting concentration and how many units each dose becomes.

For a multi-dose vial you will draw from over several days or weeks, bacteriostatic water is preferred — it contains 0.9% benzyl alcohol that suppresses bacterial growth, so the vial stays usable for weeks in the fridge. Sterile water and saline have no preservative and are really only suited to a single same-day use. The calculator’s maths is identical whichever diluent you use — the difference is shelf life, not concentration.

Doses per vial = total peptide ÷ dose per injection. A 10 mg (10,000 mcg) vial dosed at 250 mcg gives 40 doses; at 500 mcg it gives 20. The reconstitution water does not change the number of doses — only the volume of each draw. The calculator shows doses per vial and, if you set a frequency, how many days or weeks that lasts.

Exactly the same way as any peptide — GLP-1 medications are just dosed in milligrams. Enter the vial size (for example 5 mg semaglutide or 30 mg tirzepatide), the bacteriostatic water, and your weekly dose in mg. At 5 mg semaglutide in 2 ml (2.5 mg/ml), a 0.25 mg starting dose is 0.1 ml = 10 units; at 30 mg tirzepatide in 3 ml (10 mg/ml), a 2.5 mg dose is 0.25 ml = 25 units. Always follow your prescriber’s titration schedule — these drugs are prescription-only.

It depends on the concentration you want. For a 10 mg retatrutide vial, 1 ml gives 10 mg/ml and 2 ml gives 5 mg/ml; for 20 mg, 2 ml gives 10 mg/ml; for 30 mg, 3 ml gives 10 mg/ml. Keeping the vial at 10 mg/ml is popular because a 2 mg dose is a tidy 0.2 ml = 20 units. See the bacteriostatic-water chart on this page for every common vial size, or use the reverse solver in the Converters tab.

Milligrams (mg) and micrograms (mcg) measure the amount of peptide — 1 mg = 1,000 mcg. Millilitres (ml) measure the volume of liquid you draw. Concentration links them: mg/ml tells you how much peptide is in each millilitre. You dose in mg or mcg, but you draw in ml (and read it as syringe units). Mixing the units up is the single most common dosing mistake — the calculator keeps them straight for you.

The U number is how many units fit in 1 ml. A U-100 syringe (the most common) reads 100 units per ml, so 1 unit = 0.01 ml. A U-40 reads 40 units per ml (1 unit = 0.025 ml) and a U-50 is a half-ml U-100 barrel. The volume you inject is the same on any syringe — only the unit markings differ. Set your syringe type in the calculator so the unit reading matches your barrel.

For recombinant human growth hormone the accepted conversion is 1 mg ≈ 3 IU (so 1 IU ≈ 0.333 mg = 333 mcg). Switch the vial and dose units to IU in the calculator and it works entirely in IU; the IU-per-mg factor is adjustable if your product states a different potency. Most other peptides are dosed in mg/mcg and do not use IU at all.

The arithmetic is exact for the numbers you enter — concentration, volume, units and doses per vial are simple ratios with no rounding until display. Real-world accuracy then depends on measuring the bacteriostatic water precisely and on your vial actually containing its stated amount. Draw water slowly, read the syringe at eye level, and remember a little liquid always stays in the needle and vial, so you may get very slightly fewer doses than the theoretical maximum.

Completely free — no sign-up, no download, no usage limits and no watermark. It runs entirely in your browser with plain JavaScript, so every vial size, dose and price you type stays on your device and is never uploaded. That also means it works offline-fast on any phone, which is why you don’t need a separate peptide calculator app.

Yes. The peptide dosing chart on this page lists typical vial sizes, reconstitution volumes, reference doses and frequencies for 20 common peptides, and the calculator turns any of them into exact syringe units. You can copy your results to paste into a notes app as a personal cheat sheet. The chart shows commonly-cited reference ranges for education — it is not a prescription.

Once mixed with bacteriostatic water and kept refrigerated (2–8 °C), most peptides stay stable for roughly 3–8 weeks; the benzyl alcohol in BAC water is what buys that shelf life. Keep vials out of light, never freeze a reconstituted vial repeatedly, and discard if the solution turns cloudy or shows particles. Lyophilised (freeze-dried) powder, unopened, lasts far longer — often a year or more frozen.

Peptides such as BPC-157 and TB-500 are usually given as small subcutaneous injections into the fat of the abdomen (a couple of inches from the navel), using a short insulin needle at a 45–90° angle. Some users inject subcutaneously near an injury site. Injection technique and site selection should be confirmed with a qualified healthcare professional — this tool only calculates the dose volume, not how to administer it.

No. BPC-157 and TB-500 are research peptides that are not approved by the FDA for human use, and both are prohibited in competitive sport by WADA. GLP-1 drugs like semaglutide and tirzepatide are FDA-approved but prescription-only. This calculator is an educational dosing-math tool and is not an endorsement to use any particular substance — always consult a licensed healthcare provider and follow the laws where you live.

Yes — every one of those is in the preset library, and the calculator works for any peptide because the maths is the same regardless of the molecule. Select the peptide (or a CJC-1295 + ipamorelin blend in the Blend tab), enter your vial size and water, and read the units. If your peptide isn’t listed, just type its milligrams and dose manually.

A reconstitution calculator focuses on step one — turning vial size and bacteriostatic water into a concentration (mg/ml). A dosage calculator focuses on step two — turning that concentration and your target dose into a volume and syringe units. This tool is both: it reconstitutes and doses in a single screen, and adds doses-per-vial, vial duration and cost per dose on top.